One system, one workflow, powerful sequencing applications  10xGenomicsThe company has launched the latestChromium™ System. Utilizing millions of unique resourcesDNASequence taggingGEMs，Chromium™ The system can reveal important genomic information.

1Fully functional instrument10xChromiumControllerFeaturing a retractable tray and touchscreen interface, it occupies minimal space and does not exceed1Square feet, yet able to efficiently convert10Wan to100The automated pipetting process involves tens of thousands of steps, utilizing advanced microfluidic systems to separate samples at high throughput and label them with sequence tags, ultimately achieving the goal of high-throughput detection.10xChromiumControllerGenomic analysis can be conducted, as well as functional analysis at the single-cell level.

2Single cell instrument10xChromiumSingleCellControllerFeaturing a retractable tray and touchscreen interface, it occupies minimal space and does not exceed1Square feet, yet able to efficiently convert10Wan to100The automated pipetting process involves tens of thousands of steps, utilizing advanced microfluidic systems to achieve high-throughput separation and labeling of samples with sequence tags, achieving the goal of high-throughput detection.10xChromiumSingleCellControllerOnly single-cell level functional analysis can be performed.

Improving assembly parameters: Combining this technology results in better assembly performance compared to using it aloneIlluminaThe data can be increased by up to ten times;

Universality of sequencing platform: Libraries constructed using this technology can be compatible withIlluminaThe sequencing system is perfectly matched;

Molecular fragment length: Analysis can shortenReadsAssemble and grow50-100Kboflinked-reads；

Haploid analysis: can achieve chromosome levelPhasingObtain accurate haplotype information.

1. Assign samples to100,000sreach1000,000sMicro reaction systems, each containing a specific type ofDNASequence tagging.
2.containbarcodeThe gel beads of information are first mixed with the mixture of sample and enzyme, and then mixed with the micro fluid“Double Cross”The oil surfactant solution in the connection is combined.
3.GEMs (A mixture of oil droplets containing samples, enzymes, and gel beads) flows into the reservoir and is collected. Dissolution and release of gel beadsbarcodeSequence, start labeling the sample. Each droplet containsbarcodeThe mixture of information products. Then construct a standard sequencing library.

1.Genome assembly:utilizeGemCodePlatform amplifies and introduces long fragment sequencesbarcodeSequence and sequencing adapter primers are used to break the sequence into fragments of suitable sequencing size for sequencingbarcodeThe sequence information will be multipleReadsAssemble to obtain a span in30-100Kboflinked-readsInformation is used to obtain large fragments of genetic information.

Advantages:
The library constructed through this technology can be combined withIlluminaThe sequencing system is perfectly matched, making it shortReadsAssemble and grow100KbFragments;
After combining this technology, the assembly effect is better when used aloneIlluminaThe data can be increased by up to ten times;
High precision, low cost, and easy to operate.

2.Single cell transcriptome sequencing:be based on10x GenomicsnewestChromium ™The system utilizes a micro reaction system of oil in water to distinguish different cells in a population through sequence labels, obtaining a digital gene expression profile at the single-cell level, which can achieve analysis of thousands or even tens of thousands of single-cell populations and solve conventional problemsscRNA-seqThe shortcomings of the method in terms of flux or scalability open up new ideas for single-cell research.

Advantages:
Ultra high throughput: The highest number of cell detections per sample can reach1000-10000；
Short project cycle: completed within one day from cell suspension tocDNAAll sequencing preparations for library construction;
High cell capture efficiency: Single cell capture efficiency can reach up to65%Can accurately identify rare cell types;
True single-cell sequencing: through oil droplets-barcode-The correspondence between single cells enables true single-cell sequencing;
Wide compatibility of sequencing platforms: compatible withIlluminaThe platforms of various models are highly compatible.

gDNA
Fragment requirement: Main tape>50kb 50kb~100kb insertion fragment Illumina PE150
Sequencing depth≥100X

applicationEvaluation of the effectiveness of 10x Genomics assisted genome assembly

Currently, the human genomeThe successful case of de novo sequencing and assembly is the combination of Illumina short fragment sequencing, PacBio single-molecule real-time sequencing technology, and BioNano optical profiling technology, but PacBio's sequencing cost is relatively high in this method.
This study provides a genomeA new method for de novo sequencing and assembly. This method uses 10x Genomics linked reads to obtain medium to long fragment data, and then Illumina sequencing is used in combination with BioNano to construct a genome map. The contigs are anchored and scaffold, and the final assembled genome scaffold N50 reaches 33.5Mb. And the feasibility of this method was verified by genome de novo assembly of human HapMap sample (NA12878).
useThe results of genome assembly using the 10x Genomics+Illumina+BioNano method showed that both the scaffold N50 length and the assembled genome length were significantly increased, while the number of scaffolds was significantly reduced.

Yulia Mostovoy, Michal Levy-Sakin, et al., A hybrid approach for de novo human genome sequence assembly and phasing. Nature Methods. 2016 May 9. doi: 10.1038/NMETH.3865.